Techniques in Neuroscience

Coursework essay titlesBefore starting on your essay question please read the essay guidance carefully.Use information provided in the module plus your own research to answer ONE of thequestions below.The word limit for your essay is 1500 words + 10%, that is, 1650 words. The penalty forexceeding this limit is a deduction of 5 marks, and words beyond this will not be considered.The word count includes everything in the main body of the text – headings, tables plus tablelegends, figures plus figure legends, in text citations. We do not allow footnotes orappendices. The list of references (bibliography) included at the end of your essay is notincluded in the word count.1. Dr Bradshaw’s research group wants to study whether protein P is expressed inproliferating cells of the dentate gyrus. At present no antibody for protein P isavailable. Use your knowledge of immunohistochemistry gained from the modulecontent AND your independent study to discuss experiments that will allow theresearchers to answer the questions below. Provide ONE experiment for eachquestion.(a) How could the researchers Identify the cell-type(s) within the dentate gyrus thatexpress protein P?(b) How could the researchers confirm that the protein P expressing cells are adultneural stem cells?(c) How could protein P expression be used to evaluate the effects of diet OR exerciseon adult hippocampal neurogenesis in the mouse?2. Consider a situation where human inducedpluripotential cell (iPSC) lines have beengenerated from individuals with a neuropsychiatric disorder of your choice (forexample, schizophrenia). Explain and critically evaluate the potential benefits of usinghuman iPSCs over other methodologies for studying this disorder. What type of neuralcell would you differentiate the iPSCs intoinorder to better understand thisneuropsychiatric disorder and why?3. Many major breakthroughs in our understanding and treatment of disease have beenpossible because of studies using animal models. Debate the advantages and limitationsof using animal models to understand diseases of the human brain. Discuss in youranswer two examples where such studies have proved beneficial and at least oneexample where they have failed to deliver the desired outcomes.

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